Achievements

Salient research findings/highlights

Patent Granted or Published/ Technology Developed:

Biochemical assay to estimate milk urea nitrogen in milk for the detection of mastitis(Patent: India:316057 dt. 16.07.2019)
An in-vitro method for detection of postpartum anestrous condition in buffalo( Patent no2940/DEL/2013:In collaboration with CIRB-ICAR).
Urine based pregnancy detection method for ruminant livestock animals. (Patent:Application No.202011013074 A:In collaboration with CIRB-ICAR).
Biochemical assay to estimate magnesium concentration in milk for the detection of mastitis(Patent: India:346210 dt. 07.09.2020)
Switching system operating automatically based on real time light intensity in all-weather condition (Patent applied: TEMP/E1/65195/2022- DEL).

A1/A2 β-casein Genotyping:

A total of 65 Murrah buffaloes tested for A1/A2 genotype of beta casein gene, all were found to be having A2A2 genotype.

Novel Method of Proteins Purification:

Developed a simple and rapid technique for purification of anionic proteins from a complex mixture of proteins. The technique so developed could be utilized to segregate cationic and anionic proteins. Using this technique, we succeeded in purification of the beta-casein from whole milk of buffaloes and cattle nearly up to 90%.

Rumen microbial metagenomics in relation to Residual Feed Intake.

The studies on residual feed intake (RFI) and rumen microbial metagenomics can strengthen our ability to either select, or adapt, host/ or gut microbial contributions to the efficient conversion of feedstuffs so improving productivity, product quality and environment stability.
Comparative rumen microbial metagenomics studies in low and high RFI in Murrah buffaloes are helpful to characterize general microbial diversity and the repertoire of microbial genes present, including genes associated with RFI.

Study of plant/candidate feed supplement to reduce methane production without affecting productivity in buffaloes.

In vitro rumen fermentation studies:
Locally available plants and molecules have been identified as a potential mitigator of methane emission by ruminants, through their efficacy studies during in-vitro trial with respect to change in fermentation pattern, gas production and rumen bacterial profiles.

Metabolic and Endocrine Profile of Growing Goat Kids:

A significant decrease in glucose and cholesterol concentrations was observed in both male and female kids after three months of age till 12 months.
Triglyceride and protein concentration didn’t change due to advancement of age in both male and female goats.
Estradiol concentration in both male and female kids decreased during first three months of age and then increased at six months of age.
Thyroxine hormone concentration in male gradually increased with age reaching peak at six months of age while in female it decreased at one month of age and thereafter gradually increased upto six months of age.
A gradual decrease in lymphocyte percent in goat kids with increasing age was observed till 12 months of age.
Progesterone concentration was higher at -30 day prepartum and lowest at zero day (kidding day).
Triglyceride and urea concentration was higher in twin producing goats during last month of pregnancy.

Applied Reproductive Physiology:

When sperms were incubated at 37 C, it resulted in significant decrease in progressive sperm motility, Percent live spermatozoa, percent spermatozoa with intact acrosome and superoxide dismutase.
There was significant increase in the level of lipid peroxidation in sperms at 3 hours of incubation at 37ºC.
Sperm motility, percent sperm liveability and fructose concentration were found to be positively correlated with the zinc level in the seminal plasma, whereas fructose level was found to be negatively correlated.
Administration of dinoprost tromethamine (prostaglandin F2α) intramuscularly at the time of artificial insemination increased the conception rate in buffaloes.
Supplementation of dinoprost tromethamine (prostaglandin F2α) intramuscularly at the time of artificial insemination was observed to be more effective in primiparous animals and heifers as compared to pluriparous animals.
Triu B intravaginal implant and Crestar ear implant both were found to be effective in inducing estrus response in anestrus buffalo cows. However, Triu B intravaginal implant was more effective than Crestar ear implant for the treatment of anestrus animals in terms of conception rate.

List of Ph.D. Students: